Single molecule tools Super-resolution, particle tracking, multiparameter and force based methods by Nils G. Walter

Cover of: Single molecule tools | Nils G. Walter

Published by Academic Press/Elsevier in San Diego, CA .

Written in English

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Subjects:

  • Fluorescence Spectrometry,
  • Luminescence,
  • Luminescent probes,
  • Molecular Biology,
  • Science,
  • Luminescence spectroscopy,
  • Fluorescent probes,
  • Natural history,
  • Fluorescence Resonance Energy Transfer,
  • Biosensing Techniques,
  • Laboratory Manuals,
  • Life Sciences,
  • Fluorescence,
  • Biosensors,
  • Fluorescence spectroscopy,
  • Molecular Probes,
  • SCIENCE

Edition Notes

Book details

Other titlesSuper-resolution, particle tracking, multiparameter and force based methods
Statementedited by Nils G. Walter
SeriesMethods in enzymology -- v. 475
ContributionsScienceDirect (Online service)
Classifications
LC ClassificationsQP601 .M49eb vol. 475
The Physical Object
Format[electronic resource] /
ID Numbers
Open LibraryOL25542313M
ISBN 100123814839, 0123814820
ISBN 109780123814838, 9780123814821
OCLC/WorldCa689052344

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Single Molecule Tools, Part A: Fluorescence Based Approaches (ISSN Book ) - Kindle Single molecule tools book by Walter, Nils G. Download it once and read it on your Kindle device, PC, phones or tablets. Use features like bookmarks, note taking and highlighting while reading Single Molecule Tools, Part A: Fluorescence Based Approaches (ISSN Book ).Manufacturer: Academic Press.

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Book chapter Full text access Chapter 2 - Azide-Specific Labeling of Biomolecules by Staudinger–Bertozzi Ligation: Phosphine Derivatives of Fluorescent Probes Suitable for Single-Molecule Fluorescence Spectroscopy.

Abstract. Single-molecule methods have emerged as powerful tools in life science research. These techniques allow the detection and manipulation of individual biological molecules and investigate, with unprecedented resolution, their conformations and dynamics at the nanoscale by: 2.

Thomas Basché is the editor of Single-Molecule Optical Detection, Imaging and Spectroscopy, published by Wiley. William Esco Moerner is an American physical chemist and chemical physicist with current work in the biophysics and imaging of single molecules.

He is credited with achieving the first optical detection and spectroscopy of a single molecule in condensed phases, along with his. A single-molecule experiment is an experiment that investigates the properties of individual -molecule studies may be contrasted with measurements on an ensemble or bulk collection of molecules, where the individual behavior of molecules cannot be distinguished, and only average characteristics can be measured.

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* Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines.

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of.

Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule : $ Single-molecule experiments have provided fresh perspectives on questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and living volume is designed to further contribute to the rapid.

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe the unseen molecular Read more.

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The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic.

§ Member of the Organizing Committee for The Promise of Single Cell and Single Molecule Analysis Tools to Advance Dr. Kaminski authored more than research papers, review articles and book chapters and has given numerous invited talks at national and International conferences.

The Promise of Single-Cell and Single-Molecule Analysis Tools to Advance Environmental Health Research Proceedings of a Workshop—in Brief. Over the past decade, single-cell and single-molecule technologies have rapidly advanced. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them.

Coverage includes: Experimental techniques to monitor and manipulate individual biomolecules ; The use of single-molecule techniques in super-resolution and functional.

Do-it-yourself guide: how to use the modern single-molecule toolkit Nils G Walter, Cheng-Yen Huang, Anthony J Manzo & Mohamed A Sobhy Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences.

The stochastic imaging technique combining single particle tracking and PALM (sptPALM) has been utilized to image membrane proteins at 20 frames per second in living cells, mapping single molecule diffusion of up to thousands of molecules in the plasma membrane, and creating a spatially resolved map of single molecule diffusion coefficients Cited by: Single Molecule Detection, Analysis, and Manipulation Nils G.

Walter University of Michigan, Ann Arbor, USA 1 Introduction 1 2 History and Breadth of Applications 1 3 Preparation and Handling of the FEW 2 Preparation of Single Fluorescent Molecules or Particles 3 Immobilization or Trapping of Single Molecules for Fluorescence Detection 3.

Single molecule fluorescence dyes. An ideal fluorophore for single molecule studies must be bright (extinction coefficient, ε > 50, M −1 cm −1; quantum yield, QY > ), photostable with minimal photophysical/chemical and aggregation effects, small and water soluble with sufficient forms of bio-conjugation chemistries.

In addition, an excellent smFRET pair should have (1) large Cited by: Single-Molecule Techniques: A Laboratory Manual fills this requirement—it is the first to take researchers who know nothing about single–molecule analyses to the point where they can successfully design and execute appropriate experiments.

Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics. Single Molecule Science | The observation and manipulation of individual molecules is one of the most exciting developments in modern molecular science.

Single Molecule Science: Physical Principles and Models provides an introduction to the mathematical tools and physical theories needed to understand, explain, and model single-molecule observations.

Single molecule fluorescence resonance energy transfer (or smFRET) is a biophysical technique used to measure distances at the nanometer scale in single molecules, typically is an application of FRET wherein a pair of donor and acceptor fluorophores are excited and detected on a single molecule level.

In contrast to "ensemble FRET" which provides the FRET signal of a high. We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules.

This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.

The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule : Elsevier Science. Single Molecule Science: Physical Principles and Models provides an introduction to the mathematical tools and physical theories needed to understand, explain, and model single-molecule observations.

This book explains the physical principles underlying the major classes of single-molecule experiments such as fluorescence measurements, force. 1 A practical guide to single-molecule FRET Rahul Roy, Sungchul Hohng & Taekjip Ha Supplementary figures and text: Supplementary Table 1: List of Materials and Reagents.

Supplementary Table 2: List of Optics and Instruments. Supplementary Protocol: Polyethylene Glycol (PEG) surface passivation protocol. Supplementary Methods: TIR excitation and emission Size: KB.

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Single Molecule Science: Physical Principles and Models provides an introduction to the mathematical tools and physical theories needed to understand, explain, and model single-molecule observations. This book explains the physical principles underlying the major classes of single-molecule experiments such as fluorescence measurements, force.

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Robert Dunn, Chem. Rev.vol, File Size: 2MB. Single-Molecule Analysis of Nucleic Acids and DNA-Protein Interactions Using Nanopores M. Wanunu and A. Meller OTHER TECHNIQUES Single-Molecule Gold-Nanoparticle Tracking with High Temporal and Spatial Resolution and without an Applied Load A.R.

Dunn and J. Spudich Advances in Surface-based Assays for Single MoleculesPrice: $   This article reviews the experimental techniques of single-molecule fluorescence spectroscopy and microscopy with emphasis on studies at room temperature where the same single molecule is studied for an extended period.

Key to successful single-molecule detection is the need to optimize signal-to-noise ratio, and the physical parameters Cited by: Member of the Organizing Committee for The Promise of Single Cell and Single Molecule Analysis Tools to Advance Environmental Health Research.

† Member of the Standing Committee on the Use of Emerging Science for Environmental Health Decisions (ESEHD) Ph.D. at the University of Michigan, all in mechanical engineering. He completed his postdoc in. Molecules, an international, peer-reviewed Open Access journal.

Dear Colleagues, Single molecule experiments have unraveled processes that were previously concealed in bulk experiments because they were not synchronized or even heterogeneously distributed among individual molecules in a population.

Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the Cited by: Single- molecule methods are allowing us to do just that2,3.

They may one day become an elementary tool for character-izing proteins, signaling pathways or any biological phe-nomenon. In the hopes of facilitating this objective, we provide a brief but practical guide for single-molecule fluorescence resonance energy transfer (smFRET) mea.

In this chapter, we will highlight concepts to achieve superresolution by single molecule imaging, summarize tools for data analysis, and review applications on artificial and live cell membranes. Biomembranes are more than just a cell's envelope - as the interface to the surrounding of a cell they carry key signalling by: 7.Single molecule techniques promise novel information about the properties and behavior of individual particles, thus enabling access to molecular heterogeneities in biological systems.Title:Single-molecule Real-time (SMRT) Isoform Sequencing (Iso-Seq) in Plants: The Status of the Bioinformatics Tools to Unravel the Transcriptome Complexity.

VOLUME: 14 ISSUE: 7. Author(s):Yubang Gao, Feihu Xi, Hangxiao zhang, Xuqing Liu, Huiyuan Wang, Liangzhen zhao, Cited by: 1.

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